Statistics for occurrence #1 of “Major, Surgeon” in chapter 5 of Thomas Wentworth Higginson, Massachusetts in the Army and Navy during the war of 1861-1865, vol. 2:

... Assistant Surgeon, 51st Infantry, M. V. M., in service of the U. S., Nov. 25, 1862. Mustered out, July 27, 1863. First Lieutenant, Assistant Surgeon, 30th Mass. Infantry, Aug. 11, 1862. Major, Surgeon , Dec. 27, 1864. Mustered out, July 5, 1866. First Lieutenant, Assistant Surgeon, 34th Mass. Infantry, Mar. 21, 1864. First Lieutenant, Assistant Surgeon, 58th Mass. Infantry, Apr. 12, 1864...
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