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[589] collection of all the species found in the great estuary ought to be made with the view of ascertaining how far marine Atlantic species penetrate into the river basin; then one from Santarem, and another either from Talavera or Toledo or Aranjuez, and one from the head-waters in Guadalaxara, and another in Molina.

The collections made at different stations ought carefully to be kept in distinct jars or kegs, with labels so secure that no confusion or mistake can arise. But the specimens collected at the same station may be put together in the same jar. These collections require, in fact, very little care. (Here some details about mode of putting up specimens, transportation, etc.) If the same person should collect upon different stations, either in the same or in different hydrographic basins, the similarity of the specimens should not be a reason for neglecting to preserve them. What is aimed at is not to secure a variety of species, but to learn in what localities the same species may occur again and again, and what are the localities which nourish different species, no matter whether these species are in themselves interesting or not, new to science or known for ages, whether valuable for the


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